|
KMID : 0613820100200020269
|
|
Journal of Life Science
2010 Volume.20 No. 2 p.269 ~ p.274
|
|
|
Antifungal Activity of Bacillus sp. BCNU 2003 against the Human Pathogenic Fungi
|
|
Choi Hye-Jung
Yang Uk-Hee
Kim Ya-Ell
Chol Yeon-Hee
Ahn Cheol-Soo
Jeong Yong-Kee
Kim Dong-Wan
Joo Woo-Hong
|
|
|
Abstract
|
|
|
|
An antifungal antibiotic-producing strain, BCNU 2003, was isolated from forest soil in Korea. The morphological and physiological characters, and 16S rRNA sequences analysis of strain BCNU 2003 identified this strain as Bacillus genus. The Bacillus sp. BCNU 2003 showed strong antifungal activities against Aspergillus niger, Trichophyton mentagrophytes and Trichophyton rubrum with inhibition ranging from 62.05 to 63.49% by using dual culture technique. Bacillus sp. BCNU 2003 produced a maximum level of antifungal substances under aerobic incubation at 28℃ and pH 6.5-7.2 for 6 days in LB broth. Ethyl acetate extract of the cultured broth showed strong antifungal activity and a broad antifungal spectrum against various pathogenic fungi. The minimum inhibitory concentration (MIC) values for its active extracts ranged between 0.0625 ㎎/ml and 1 ㎎/ml. In addition, Bacillus sp. BCNU 2003 was determined to have the ability to produce enzymes such as amylase, protease, gelatinase and catalase.
|
|
|
KEYWORD
|
|
|
Antifungal activity, human pathogenic fungi, Bacillus sp. BCNU 2003, Bacillus sp., minimal inhibitory concentration
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|